Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized Powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- Peptide (C)EDKEMAPEKWYSLGK, corresponding to amino acid residues 653 - 667 of human L1CAM (Accession P32004).
Accession (Uniprot) Number P32004
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain membrane (lanes 2 and 4):1-2. Anti-L1CAM (extracellular) Antibody (#ANR-047), (1:200).
3-4. Anti-L1CAM (extracellular) Antibody, preincubated with L1CAM (extracellular) Blocking Peptide (BLP-NR047).
Western blot analysis of human MCF-7 breast adenocarcinoma cell line lysate (lanes 1 and 3) and human THP-1 monocytic leukemia cell line lysate (lanes 2 and 4):1-2. Anti-L1CAM (extracellular) Antibody (#ANR-047), (1:200).
3-4. Anti-L1CAM (extracellular) Antibody, preincubated with L1CAM (extracellular) Blocking Peptide (BLP-NR047).
Last update: 02/01/2024
