Overview
Type: Synthetic peptide
Form: Lyophilized powder
LAT3/SLC43A1 (extracellular) Blocking Peptide (#BLP-NT193) is the original antigen used for immunization during Anti-LAT3/SLC43A1 (extracellular) Antibody (#ANT-193) generation. The blocking peptide binds and ‘blocks’ Anti-LAT3/SLC43A1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat lung (lanes 1 and 3) and mouse lung (lanes 2 and 4) lysates:1, 2. Anti-LAT3/SLC43A1 (extracellular) Antibody (#ANT-193), (1:400).
3, 4. Anti-LAT3 (SLC43A1) (extracellular) Antibody, preincubated with LAT3/SLC43A1 (extracellular) Blocking Peptide (#BLP-NT193).
Western blot analysis of human chronic myelogenous leukemia K562 (lanes 1 and 3) and human prostate carcinoma LN-CaP (lanes 2 and 4) cell lines lysates:1, 2. Anti-LAT3 (SLC43A1) (extracellular) Antibody (#ANT-193), (1:200).
3, 4. Anti-LAT3 (SLC43A1) (extracellular) Antibody, preincubated with LAT3/SLC43A1 (extracellular) Blocking Peptide (#BLP-NT193).
Properties
Sequence
- (C)GKERETNEQRQKVE, corresponding to amino acid residues 336-349 of mouse LAT3 (SLC43A1) (Accession Q8BSM7).
Accession (Uniprot) Number Q8BSM7
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
