Overview
Type: Synthetic peptide
Form: Lyophilized powder
Latrophilin-1/LPHN1 (extracellular) Blocking Peptide (#BLP-LR021) is the original antigen used for immunization during Anti-Latrophilin-1 (LPHN1) (extracellular) Antibody (#ALR-021) generation. The blocking peptide binds and ‘blocks’ Anti-Latrophilin-1/LPHN1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-Latrophilin-1 (LPHN1) (extracellular) Antibody (#ALR-021), (1:200).
3,4. Anti-Latrophilin-1 (LPHN1) (extracellular) Antibody, preincubated with Latrophilin-1/LPHN1 (extracellular) Blocking Peptide (#BLP-LR021).
Expression of Latrophilin-1 receptor in rat hippocampusImmunohistochemical staining of rat hippocampal dentate granule layer (G) using Anti-Latrophilin-1 (LPHN1) (extracellular) Antibody (#ALR-021). A. Latrophilin-1 receptor staining (red) appears in astrocytes. B. Nuclear staining using DAPI as the counterstain (blue). C. Merged image of A and B.
Properties
Sequence
- CEPREVRRVQWPATQ(G), corresponding to amino acid residues 480-494 of rat Latrophilin-1 (Accession O88917).
Accession (Uniprot) Number O88917
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
