Overview
Type: Synthetic peptide
Form: Lyophilized powder
LETM1 Blocking Peptide (#BLP-NT183) is the original antigen used for immunization during Anti-LETM1 Antibody (#ANT-183) generation. The blocking peptide binds and ‘blocks’ Anti-LETM1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membrane (lanes 1 and 5), mouse brain membrane (lanes 2 and 6), human acute lymphoblastic leukemia (MOLT-4) (lanes 3 and 7) and human colorectal adenocarcinoma (HT-29) cell lysates:1-4. Anti-LETM1 Antibody (#ANT-183), (1:1500).
5-8. Anti-LETM1 Antibody, preincubated with LETM1 Blocking Peptide (#BLP-NT183).
Properties
Sequence
- (C)KELSKTGDEKYIEE, corresponding to amino acid residues 587-600 of rat LETM1 (Accession Q5XIN6).
Accession (Uniprot) Number Q5XIN6
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
