Overview
Type: Synthetic peptide
Form: Lyophilized powder
LINGO-1 (extracellular) Blocking Peptide (#BLP-NT032) is the original antigen used for immunization during Anti-LINGO-1 (extracellular) Antibody (#ANT-032) generation. The blocking peptide binds and ‘blocks’ Anti-LINGO-1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-LINGO-1 (extracellular) Antibody (#ANT-032), (1:200).
3,4. Anti-LINGO-1 (extracellular) Antibody, preincubated with LINGO-1 (extracellular) Blocking Peptide (#BLP-NT032).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:1. Anti-LINGO-1 (extracellular) Antibody (#ANT-032), (1:500).
2. Anti-LINGO-1 (extracellular) Antibody, preincubated with LINGO-1 (extracellular) Blocking Peptide (#BLP-NT032).
Expression of LINGO-1 in rat brain and spinal cordImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-LINGO-1 (extracellular) Antibody (#ANT-032), (1:400), followed by donkey-anti-rabbit-Cy3. A. LINGO-1 staining (red) is detected in sections of rat substantia nigra, in nigral neurons (arrows). B. Staining of rat spinal cord shows LINGO-1 labeling in ventral horn neurons (arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)HVRSYSPDWPHQPNK, corresponding to amino acid residues 507-521 of mouse LINGO-1 (Accession Q9D1T0).
Accession (Uniprot) Number Q9D1T0
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
