Overview
Type: Synthetic peptide
Form: Lyophilized powder
LPAR4/P2Y9 (extracellular) Blocking Peptide (#BLP-LR034) is the original antigen used for immunization during Anti-LPAR4 (P2Y9) (extracellular) Antibody (#ALR-034) generation. The blocking peptide binds and ‘blocks’ Anti-LPAR4/P2Y9 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) heart membranes:1,2. Anti-LPAR4 (P2Y9) (extracellular) Antibody (#ALR-034), (1:500).
3,4. Anti-LPAR4 (P2Y9) (extracellular) Antibody, preincubated with LPAR4/P2Y9 (extracellular) Blocking Peptide (#BLP-LR034).
Expression of LPA4 receptor in rat and mouse brainImmunohistochemical staining of rat choroid plexus and mouse hippocampal CA3 region using Anti-LPAR4 (P2Y9) (extracellular) Antibody (#ALR-034), (1:400). A. LPA4 receptor staining (red) in rat choroid plexus appears in astrocytes (vertical arrows) and in cells of the choroid plexus (horizontal arrow). C. LPA4 receptor staining (red) in mouse hippocampal CA3 region is detected in astrocytes (arrows). B, D. Nuclear staining using DAPI as the counterstain (blue).
Properties
Sequence
- (C)DRRFIDFQFQDLNS, corresponding to amino acid residues 3-16 of mouse LPA4 receptor (Accession Q8BLG2).
Accession (Uniprot) Number Q8BLG2
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
