Overview
Type: Synthetic peptide
Form: Lyophilized Powder
LRRC33 (extracellular) Blocking Peptide (#BLP-LR043) is the original antigen used for immunization during Anti-LRRC33 (extracellular) Antibody (#ALR-043) generation. The blocking peptide binds and ‘blocks’ Anti-LRRC33 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):1-2. Anti-LRRC33 (extracellular) Antibody (#ALR-043), (1:200).
3-4. Anti-LRRC33 (extracellular) Antibody, preincubated with LRRC33 (extracellular) Blocking Peptide (BLP-LR043).
Western blot analysis of human THP-1 monocytic leukemia cell line lysate:1. Anti-LRRC33 (extracellular) Antibody (#ALR-043), (1:400).
2. Anti-LRRC33 (extracellular) Antibody, preincubated with LRRC33 (extracellular) Blocking Peptide (BLP-LR043).
Expression of LRRC33 in rat fornix.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-LRRC33 (extracellular) Antibody (#ALR-043), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. LRRC33 immunoreactivity (green) appears in glial outlines (arrows). B. Pre-incubation of the antibody with LRRC33 (extracellular) Blocking Peptide (BLP-LR043), suppressed staining. Cell nuclei are stained with DAPI (blue). LV= Lateral ventricle; FX= Fornix.
Properties
Sequence
- Peptide (C)HIREHEPPGALTELD, corresponding to amino acid residues 368 - 382 of mouse NRROS (Accession Q8BMT4).
Accession (Uniprot) Number Q8BMT4
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
