Overview
Type: Synthetic peptide
Form: Lyophilized powder
LRRC8A (extracellular) Blocking Peptide (#BLP-AC001) is the original antigen used for immunization during Anti-LRRC8A (extracellular) Antibody (#AAC-001) generation. The blocking peptide binds and ‘blocks’ Anti-LRRC8A (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain lysate:1. Anti-LRRC8A (extracellular) Antibody (#AAC-001), (1:200).
2. Anti-LRRC8A (extracellular) Antibody, preincubated with LRRC8A (extracellular) Blocking Peptide (#BLP-AC001).
Western blot analysis of rat lung membranes:1. Anti-LRRC8A (extracellular) Antibody (#AAC-001), (1:200).
2. Anti-LRRC8A (extracellular) Antibody, preincubated with LRRC8A (extracellular) Blocking Peptide (#BLP-AC001).
Expression of LRRC8A in mouse cerebellum and olfactory bulbImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-LRRC8A (extracellular) Antibody (#AAC-001), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. LRRC8A staining in mouse cerebellum (green) shows intensely positive cells (arrows) with Bergmann glia morphology and localization. B. Staining in mouse olfactory bulb shows intensely positive cells (arrows) with glial morphology in some glomeruli (G). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)DTGPTGIKYDLDRH, corresponding to amino acid residues 91-104 of human LRRC8A (Accession Q8IWT6).
Accession (Uniprot) Number Q8IWT6
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
