Overview
Type: Synthetic peptide
Form: Lyophilized powder
Lynx1 (extracellular) Blocking Peptide (#BLP-NC021) is the original antigen used for immunization during Anti-Lynx1 (extracellular) Antibody (#ANC-021) generation. The blocking peptide binds and ‘blocks’ Anti-Lynx1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1, 2. Anti-Lynx1 (extracellular) Antibody (#ANC-021), (1:200).
3, 4. Anti-Lynx1 (extracellular) Antibody, preincubated with Lynx1 (extracellular) Blocking Peptide (#BLP-NC021).
Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and human Jurkat T-cell leukemia cell line lysate (lanes 2 and 4):1, 2. Anti-Lynx1 (extracellular) Antibody (#ANC-021), (1:200).
3, 4. Anti-Lynx1 (extracellular) Antibody, preincubated with Lynx1 (extracellular) Blocking Peptide (#BLP-NC021).
Expression of Lynx1 in rat dorsal raphe nucleus and substantia nigra pars compactaImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Lynx1 (extracellular) Antibody (#ANC-021), (1:1000), followed by goat anti-rabbit-AlexaFluor-488. A. Lynx1 staining in the dorsal raphe nucleus (DRN), (green) is detected in cells of the medial DRN (MDRN, horizontal arrows) and in cells of the lateral DRN (LDRN), (vertical arrows). B. Lynx1 immunoreactivity in the substantia nigra pars compacta (SNC), (green) is observed in cells of the SNC (arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- CAYNGDN(S)FKPMR, corresponding to amino acid residues 26-38 of mouse Lynx1 (Accession P0DP60).
Accession (Uniprot) Number P0DP60
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
