Cat #: BLP-NT197
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized Powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- Peptide (C)SAQRKKEMVLSEK, corresponding to amino acid residues 28-40 of human MAGT1 (Accession Q9H0U3).
Accession (Uniprot) Number Q9H0U3
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of mouse colon lysate (lanes 1 and 4), rat brain lysate (lanes 2 and 5) and mouse brain lysate (lanes 3 and 6):1-3. Anti-MAGT1 (extracellular) Antibody (#ANT-197), (1:200).
4-6. Anti-MAGT1 (extracellular) Antibody, preincubated with MAGT1 (extracellular) Blocking Peptide (BLP-NT197). - Western blot analysis of human HepG2 hepatocarcinoma cell line lysate (lanes 1 and 4), human Jurkat T-cell leukemia cell line lysate (lanes 2 and 5) and human
THP-1 monocytic leukemia cell line lysate (lanes 3 and 6):1-3. Anti-MAGT1 (extracellular) Antibody (#ANT-197), (1:200).
4-6. Anti-MAGT1 (extracellular) Antibody, preincubated with MAGT1 (extracellular) Blocking Peptide (BLP-NT197).
Last update: 02/01/2024