Overview
Type: Synthetic peptide
Form: Lyophilized powder
MANF/ARMET Blocking Peptide (#BLP-NT028) is the original antigen used for immunization during Anti-MANF/ARMET Antibody (#ANT-028) generation. The blocking peptide binds and ‘blocks’ Anti-MANF/ARMET primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain lysate (lanes 1 and 4), rat brain lysate (lanes 2 and 5) and human recombinant MANF protein (lanes 3 and 6):1-3. Anti-MANF/ARMET Antibody (#ANT-028), (1:200).
4-6. Anti-MANF/ARMET Antibody, preincubated with MANF/ARMET Blocking Peptide (#BLP-NT028).
Expression of MANF in rat parietal cortexImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-MANF/ARMET Antibody (#ANT-028), (1:600), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. Sections derived from parietal cortex of a rat at postnatal day 22 reveal MANF staining (green) in nuclei and cytoplasm of several cells (arrows) in deep layers of the cortex. Nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)EVSKPLAHHIPVEK, corresponding to amino acid residues 91-104 of human MANF (Accession P55145).
Accession (Uniprot) Number P55145
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
