Overview
Type: Synthetic peptide
Form: Lyophilized powder
MC2 Receptor (extracellular) Blocking Peptide (#BLP-MR022) is the original antigen used for immunization during Anti-MC2 Receptor (extracellular) Antibody (#AMR-022) generation. The blocking peptide binds and ‘blocks’ Anti-MC2 Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat adrenal lysate:1. Anti-MC2 Receptor (extracellular) Antibody (#AMR-022), (1:400).
2. Anti-MC2 Receptor (extracellular) Antibody, preincubated with MC2 Receptor (extracellular) Blocking Peptide (#BLP-MR022).
Expression of MC2R in rat adrenal glandImmunohistochemical staining of paraffin embedded rat adrenal cortex section using Anti-MC2 Receptor (extracellular) Antibody (#AMR-022), (1:100). Shown are the capsule (C), the zona glomerulosa (ZG) and the zona fasciculata (ZF) of the adrenal cortex. Note that the staining (brown) is specific for cells of the adrenal gland while no staining is present in the dense connective tissue of the capsule. Color reaction was obtained with DAB. H&E is used as the counterstain.
Properties
Sequence
- (C)RNMGYLKPRGSFE, corresponding to amino acid residues 87-99 of human MC2R (Accession Q01718).
Accession (Uniprot) Number Q01718
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
