Overview
Type: Synthetic peptide
Form: Lyophilized powder
MC3 Receptor (extracellular) Blocking Peptide (#BLP-MR023) is the original antigen used for immunization during Anti-MC3 Receptor (extracellular) Antibody (#AMR-023) generation. The blocking peptide binds and ‘blocks’ Anti-MC3 Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,2. Anti-MC3 Receptor (extracellular) Antibody (#AMR-023), (1:200).
3,4. Anti-MC3 Receptor (extracellular) Antibody, preincubated with MC3 Receptor (extracellular) Blocking Peptide (#BLP-MR023).
Expression of MC3R in rat brainImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-MC3 Receptor (extracellular) Antibody (#AMR-023), (1:100). Expression (green) appears in the hypothalamic periventricular region (horizontal arrow), in some nerve cells (vertical arrow), and in the neuropil (asterisk). Hoechst 33342 is used as the counterstain (blue).
Properties
Sequence
- (C)NSDSLTLEDQFIQHMD, corresponding to amino acid residues 102-117 of rat MC3R (Accession P32244).
Accession (Uniprot) Number P32244
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
