Cat #: BLP-MR024
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- (C)HRGMHTSLHLWNRSS, corresponding to amino acid residues 6-20 of human MC4R (Accession P32245).
Accession (Uniprot) Number P32245
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat brain lysates:1. Anti-MC4 Receptor (extracellular) Antibody (#AMR-024), (1:400).
2. Anti-MC4 Receptor (extracellular) Antibody, preincubated with MC4 Receptor (extracellular) Blocking Peptide (#BLP-MR024). - Western blot analysis of mouse brain lysates:1. Anti-MC4 Receptor (extracellular) Antibody (#AMR-024), (1:400).
2. Anti-MC4 Receptor (extracellular) Antibody, preincubated with MC4 Receptor (extracellular) Blocking Peptide (#BLP-MR024). - Mouse brain sections (frozen), (1:50).
Last update: 02/01/2024