Overview
Alternative Name Mast cell degranulating peptide, MCDP, Peptide 401
Lyophilized Powder yes
Origin Synthetic peptide
MW: 2588 Da
Purity: >98% (HPLC)
Effective concentration 10 nM - 1 μM.
Sequence IKCNCKRHVIKPHICRKICGKN.
Modifications Disulfide bonds between Cys3-Cys15, and Cys5-Cys19. Asn22 - C-terminal amidation.
Structure
Molecular formula C110H192N40O24S4.
CAS No.: 74601-64-2
Activity MCD peptide inhibits KV1.1 and KV1.2 channels1,2.
References-Activity
- Guillemare, E. et al. (1992) Biochemistry 31, 12463.
- Grissmer, S. et. al. (1994) Mol. Pharmacol. 45, 1227.
Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage of solutions Up to four weeks at 4°C or six months at -20°C.
Our bioassay
Alomone Labs MCD peptide inhibits KV1.2 channels heterologously expressed in Xenopus oocytes.Time course of KV1.2 current inhibition by 200 nM of MCD peptide (#STM-250) (orange) compared to natural MCD peptide (green) recorded at -20 mV. The toxins were perfused to the recording bath for a period of 100 sec. Holding potential was -80 mV and test pulses to 60 mV were delivered for 150 msec.
Scientific background
MCD peptide (Mast Cell Degranulating Peptide) is a 22 amino acid peptidyl toxin isolated from Apis mellifera bee venom1. MCD peptide induces histamine release from mast cells2 and release of glutamate in CA1 region of rat hippocampus in dose-dependent manner3.
MCD peptide was shown to block KV1.2 channels in Xenopus oocytes4 and to inhibit KV1.1 (IC50 490 nM) and KV1.2 (IC50 440 nM) expressed in cell lines while having no effect on KV1.3, KV1.5 and KV3.15. In accordance, MCD peptide (6.4 µM) enhances the magnitude of field potentials in CA1 region as recorded in hippocampal slices6 and induces epileptiform seizures and brain damage after intracerebroventricular injection of 200 pM in rats7.
Target KV1.1, KV1.2 K+ channels
Peptide Content: 100%
Lyophilized Powder
For research purposes only, not for human use
Last Update: 08/01/2025
Specifications
Citations
Citations
