Overview
Type: Synthetic peptide
Form: Lyophilized powder
MCT1/SLC16A1 (extracellular) Blocking Peptide (#BLP-MT011) is the original antigen used for immunization during Anti-MCT1 (SLC16A1) (extracellular) Antibody (#AMT-011) generation. The blocking peptide binds and ‘blocks’ Anti-MCT1/SLC16A1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-MCT1 (SLC16A1) (extracellular) Antibody (#AMT-011), (1:200).
3,4. Anti-MCT1 (SLC16A1) (extracellular) Antibody, preincubated with MCT1/SLC16A1 (extracellular) Blocking Peptide (#BLP-MT011).
Western blot analysis of rat skeletal muscle lysate:1. Anti-MCT1 (SLC16A1) (extracellular) Antibody (#AMT-011), (1:200).
2. Anti-MCT1 (SLC16A1) (extracellular) Antibody, preincubated with MCT1/SLC16A1 (extracellular) Blocking Peptide (#BLP-MT011).
Western blot analysis of human HT-29 colon adenocarcinoma cell line lysate (lanes 1 and 3) and human ARPE-19 retinal pigment epithelium cell line lysate (lanes 2 and 4):1,2. Anti-MCT1 (SLC16A1) (extracellular) Antibody (#AMT-011), (1:200).
3,4. Anti-MCT1 (SLC16A1) (extracellular) Antibody, preincubated with MCT1/SLC16A1 (extracellular) Blocking Peptide (#BLP-MT011).
Expression of monocarboxylate transporter 1 in mouse hippocampusImmunohistochemical staining of immersion-fixed, free floating mouse brain frozen sections using Anti-MCT1 (SLC16A1) (extracellular) Antibody (#AMT-011), (1:200), followed by goat-anti-rabbit-Cy3. A. MCT1 staining (red) in CA1 hippocampal region, is detected in the pyramidal layer (P) and in small cell outlines (arrows). B. GFAP staining (green) is observed in astrocyte outlines (arrows). C. Merge of the two images demonstrates co-localization of MCT1 and GFAP in astrocytes (arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)GRLNDMYGDYKYT, corresponding to amino acid residues 403-415 of rat MCT1 (Accession P53987).
Accession (Uniprot) Number P53987
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
