Overview
Type: Synthetic peptide
Form: Lyophilized powder
MCT2 Blocking Peptide (#BLP-MT012) is the original antigen used for immunization during Anti-MCT2 Antibody (#AMT-012) generation. The blocking peptide binds and ‘blocks’ Anti-MCT2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse testis membranes:1. Anti-MCT2 Antibody (#AMT-012), (1:200).
2. Anti-MCT2 Antibody, preincubated with MCT2 Blocking Peptide (#BLP-MT012).
Western blot analysis of rat brain lysate (lanes 1 and 3) and rat testis membranes (lanes 2 and 4):1,2. Anti-MCT2 Antibody (#AMT-012), (1:200).
3,4. Anti-MCT2 Antibody, preincubated with MCT2 Blocking Peptide (#BLP-MT012).
Expression of MCT2 in mouse brain sectionsImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-MCT2 Antibody (#AMT-012), (1:1000). A. MCT2 staining in mouse cortex sections (red) appears in neuronal outlines (arrows). B. MCT2 staining in dorsal pons brain stem regions (red) appears in neuronal outlines (horizontal arrows) and in fibers (vertical arrows). 4th V = fourth ventricle. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)KEMEALSRSKQDD, corresponding to amino acid residues 456-468 of rat MCT2 (Accession Q63344).
Accession (Uniprot) Number Q63344
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
