Overview
Type: Synthetic peptide
Form: Lyophilized Powder
MEGF10 (extracellular) Blocking Peptide (#BLP-NR205) is the original antigen used for immunization during Anti-MEGF10 (extracellular) Antibody (#ANR-205) generation. The blocking peptide binds and ‘blocks’ Anti-MEGF10 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1-2. Anti-MEGF10 (extracellular) Antibody (#ANR-205), (1:400).
3-4. Anti-MEGF10 (extracellular) Antibody, preincubated with MEGF10 (extracellular) Blocking Peptide (BLP-NR205).
Western blot analysis of human U-87 MG glioblastoma cell line lysate (lanes 1 and 4), human MCF-7 breast adenocarcinoma (lanes 2 and 5) and human 1311N1 astrocytoma cell line lysate (lanes 3 and 6):1-3. Anti-MEGF10 (extracellular) Antibody (#ANR-205), (1:400).
4-6. Anti-MEGF10 (extracellular) Antibody, preincubated with MEGF10 (extracellular) Blocking Peptide (BLP-NR205).
Expression of MEGF10 in rat substantia nigra.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-MEGF10 (extracellular) Antibody (#ANR-205), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. MEGF10 immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the antibody with MEGF10 (extracellular) Blocking Peptide (BLP-NR205), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide CEDR(S)EQGTYGND, corresponding to amino acid residues 178 - 190 of mouse MEGF10 (Accession Q6DIB5).
Accession (Uniprot) Number Q6DIB5
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
