Overview
Type: Synthetic peptide
Form: Lyophilized powder
Melatonin Receptor 1A/MTNR1A Blocking Peptide (#BLP-MR031) is the original antigen used for immunization during Anti-Melatonin Receptor 1A (MTNR1A) Antibody (#AMR-031) generation. The blocking peptide binds and ‘blocks’ Anti-Melatonin Receptor 1A/MTNR1A primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain (lanes 1 and 4), rat brain (lanes 2 and 5) and rat cerebellum (lanes 3 and 6) lysates:1-3. Anti-Melatonin Receptor 1A (MTNR1A) Antibody (#AMR-031), (1:200).
4-6. Anti-Melatonin Receptor 1A (MTNR1A) Antibody, preincubated with Melatonin Receptor 1A/MTNR1A Blocking Peptide (#BLP-MR031).
Expression of Melatonin receptor type 1A in rat supra-chiasmatic nucleusImmunohistochemical staining of rat supra-chiasmatic nucleus (SCN) with Anti-Melatonin Receptor 1A (MTNR1A) Antibody (#AMR-031). A. MT1 staining is restricted to the SCN (red). B. DAPI is used as the counterstain (blue). C. Merge of A and B.
Properties
Sequence
- (C)RVKPDNKPKLKPQD, corresponding to amino acid residues 223-236 of mouse MTNR1A (Accession Q61184).
Accession (Uniprot) Number Q61184
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
