Overview
Type: Synthetic peptide
Form: Lyophilized powder
mGluR3 (extracellular) Blocking Peptide (#BLP-GC012) is the original antigen used for immunization during Anti-mGluR3 (extracellular) Antibody (#AGC-012) generation. The blocking peptide binds and ‘blocks’ Anti-mGluR3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-mGluR3 (extracellular) Antibody (#AGC-012), (1:600).
3,4. Anti-mGluR3 (extracellular) Antibody, preincubated with mGluR3 (extracellular) Blocking Peptide (#BLP-GC012).
Expression of mGluR3 in rat brain stemImmunohistochemical staining of frozen brain stem sections using Anti-mGluR3 (extracellular) Antibody (#AGC-012), (1:100) followed by goat anti-rabbit AlexaFluor-555 secondary antibody (red). Staining is present in neuronal cell bodies (arrows) in the brainstem nuclei. Hoechst 33342 is used as the counterstain (blue).
Properties
Sequence
- (C)GDHNFMRREIKIEGD, corresponding to amino acids 24-38 of rat mGluR3 (Accession P31422).
Accession (Uniprot) Number P31422
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
