Overview
Type: Synthetic peptide
Form: Lyophilized powder
mGluR7 (extracellular) Blocking Peptide (#BLP-GC017) is the original antigen used for immunization during Anti-mGluR7 (extracellular) Antibody (#AGC-017) generation. The blocking peptide binds and ‘blocks’ Anti-mGluR7 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 4) and rat (lanes 2 and 5) brain membranes and human CCF-STTGI astrocytoma (lanes 3 and 6) cell line lysate (1:200):1-3. Anti-mGluR7 (extracellular) Antibody (#AGC-017), (1:200).
4-6. Anti-mGluR7 (extracellular) Antibody, preincubated with mGluR7 (extracellular) Blocking Peptide (#BLP-GC017).
Expression of mGluR7 in rat neocortexImmunohistochemical staining of rat neocortex frozen sections using Anti-mGluR7 (extracellular) Antibody (#AGC-017). A. mGluR7 staining (green) appears in several neocortical neurons. B. Merge image showing mGluR7 staining together with cell nuclei (blue).
Properties
Sequence
- TISGSKKEDTDRKC, corresponding to amino acid residues 377-390 of rat mGluR7 (Accession P35400).
Accession (Uniprot) Number P35400
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
