Overview
Type: Synthetic peptide
Form: Lyophilized powder
mGluR8 (extracellular) Blocking Peptide (#BLP-GC028) is the original antigen used for immunization during Anti-mGluR8 (extracellular) Antibody (#AGC-028) generation. The blocking peptide binds and ‘blocks’ Anti-mGluR8 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell line lysate (lanes 3 and 6):1-3. Anti-mGluR8 (extracellular) Antibody (#AGC-028), (1:400).
4-6. Anti-mGluR8 (extracellular) Antibody, preincubated with mGluR8 (extracellular) Blocking Peptide (#BLP-GC028).
Expression of mGluR8 in rat globus pallidusImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-mGluR8 (extracellular) Antibody (#AGC-028), (1:100). A. Staining reveals expression of mGluR8 (red) in globus pallidus neurons (arrow) and their dendrites. B. The same section was also stained with DAPI (blue) revealing cell nuclei. C. Merge of the two images.
Properties
Sequence
- (C)ENFG(S)KLGSHGKR, corresponding to amino acid residues 365-377 of rat mGluR8 (Accession P70579).
Accession (Uniprot) Number P70579
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
