Cat #: AK-535_KIT
Size: 31 Vials
Form: Lyophilized
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
Anti-CLIC4 Antibody Cat #: ACL-024
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)NGLKEEDKEPLIE, corresponding to amino acid residues 8-20 of human CLIC4 (Accession Q9Y696). Intracellular, N-terminus.
Homology
Rat, mouse - identical.
CLIC4 Blocking Peptide Cat #: BLP-CL024
Size:
40 µg
Anti-KCNK9 (TASK-3) (extracellular) Antibody Cat #: APC-044
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
H, R
Immunogen
Peptide (C)DDYQQLELVILQSEPHR, corresponding to amino acid residues 57-73 of rat KCNK9 (accession number Q9ES08). Extracellular, near the P1 loop.
Homology
Human - 15/17 amino acid residues identical.
KCNK9/TASK-3 (extracellular) Blocking Peptide Cat #: BLP-PC044
Size:
40 µg
Anti-KCNMA1 (KCa1.1) (1097-1196) Antibody Cat #: APC-021
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
M, R
Immunogen
GST fusion protein with the sequence
SHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNATRMTRMGQAEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC, corresponding to residues 1097-1196 of mouse KCNMA1 variant 2 (Accession Q08460-2). Intracellular, C-terminus.
SHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNATRMTRMGQAEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC, corresponding to residues 1097-1196 of mouse KCNMA1 variant 2 (Accession Q08460-2). Intracellular, C-terminus.
Homology
Chicken - 91/99 amino acid residues identical; rabbit - 88/99 amino acid residues identical; turtle - 87/99 amino acid residues identical.
KCNMA1/KCa1.1 (1097-1196) Blocking Peptide Cat #: BLP-PC021
Size:
0.12 mg
Guinea pig Anti-KCNMA1 (KCa1.1) (1097-1196) Antibody Cat #: APC-021-GP
Size:
50 µl
Host: Guinea pig
Type: Polyclonal
Application:
WB
Reactivity:
H, M, R
Immunogen
GST fusion protein with the sequence SHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNATRMTRMGQAEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC, corresponding to residues 1097-1196 of mouse KCNMA1 variant 2 (Accession Q08460-2). Intracellular, C-terminus.
KCNMA1/KCa1.1 (1097-1196) Blocking Peptide Cat #: BLP-PC021
Size:
0.12 mg
Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody Cat #: APC-107
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)STANRPNRPKSRESRDK, corresponding to amino acid residues 1184-1200 of mouse KCNMA1 (Accession number Q08460). Intracellular, C-terminal part.
Homology
Rat - identical; human, bovine, chicken, dog - 16/17 amino acid residues identical.
KCNMA1/KCa1.1 (1184-1200) Blocking Peptide Cat #: BLP-PC107
Size:
40 µg
Anti-KCNMA1 (KCa1.1) (extracellular) Antibody Cat #: APC-151
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IF, IFC, IH, LCI, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)DSSNPIES(S)QNFYKD, corresponding to amino acid residues 199-213 of rat KCNMA1 (Accession Q62976). 1st extracellular loop.
Homology
Rat, mouse, human - 14/15 amino acid residues identical.
KCNMA1/KCa1.1 (extracellular) Blocking Peptide Cat #: BLP-PC151
Size:
40 µg
Anti-KCNN4 (KCa3.1, SK4) Antibody Cat #: APC-064
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide RQVRLKHRKLREQV(C), corresponding to amino acid residues 350-363 of rat KCNN4 (Accession Q9QYW1). Intracellular, C-terminal part.
Homology
Mouse, human, pig - identical.
KCNN4/KCa3.1 Blocking Peptide Cat #: BLP-PC064
Size:
40 µg
Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular) Antibody Cat #: ALM-051
Size:
25 µg
Host: Mouse
Type: Monoclonal
Application:
IC, IF, IFC, IH, IP, LCI, WB
Reactivity:
H, M, R
Immunogen
Synthetic peptide mapping to the 3rd extracellular loop of human KCNN4 (Accession O15554).
Anti-KV1.3 (KCNA3) Antibody Cat #: APC-002
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
GST fusion protein with the sequence TLSKSEYMVIEEGGMNHSAFPQTPFKTGNSTATCTTNNNPNSCVNIKKIFTDV, corresponding to amino acid residues 523-575 of human KV1.3 (Accession P22001). Intracellular, C-terminus.
Homology
Rat, rabbit, mouse - identical.
Kv1.3/KCNA3 Blocking Peptide Cat #: BLP-PC002
Size:
0.12 mg
Anti-KV1.3 (KCNA3) (extracellular) Antibody Cat #: APC-101
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, IH, IP, LCI, WB
Reactivity:
H, M, R
Immunogen
Peptide KDYPASTSQDSFEA(C), corresponding to amino acid residues 263-276 of human KV1.3 (Accession P22001). Extracellular loop between domains S1 and S2.
Homology
Rat, mouse - 12/14 amino acid residues identical.
Kv1.3/KCNA3 (extracellular) Blocking Peptide Cat #: BLP-PC101
Size:
40 µg
Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody Cat #: APC-101-GP
Size:
50 µl
Host: Guinea pig
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide KDYPASTSQDSFEA(C), corresponding to amino acid residues 263-276 of human KV1.3 (Accession P22001). 1st extracellular loop.
Homology
Rat, mouse - 12/14 amino acid residues identical.
Kv1.3/KCNA3 (extracellular) Blocking Peptide Cat #: BLP-PC101
Size:
40 µg
Anti-LETM1 Antibody Cat #: ANT-183
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
WB
Reactivity:
H, M, R
Immunogen
Peptide (C)KELSKTGDEKYIEE, corresponding to amino acid residues 587-600 of rat LETM1 (Accession Q5XIN6). Intracellular, mitochondrial matrix.
Homology
Mouse –13/14 amino acid residues identical; human – 12/14 amino acid residues identical.
LETM1 Blocking Peptide Cat #: BLP-NT183
Size:
40 µg
Anti-MCUR1 (CCDC90A) Antibody Cat #: ACC-321
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
WB
Reactivity:
M, R
Immunogen
Peptide (C)GRNALGRLRLGARR, corresponding to amino acid residues 97-110 of mouse mitochondrial calcium uniporter regulator 1 (Accession Q9CXD6). Intracellular loop, mitochondrial matrix.
Homology
Rat - identical; human – no apparent homology.
MCUR1/CCDC90A Blocking Peptide Cat #: BLP-CC321
Size:
40 µg
Anti-MICU1 Antibody Cat #: ACC-322
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
WB
Reactivity:
M, R
Immunogen
Peptide (C)EFERHDPVDGRISER, corresponding to amino acid residues 313-327 of rat MICU1 (Accession Q6P6Q9). Intracellular.
Homology
Mouse - identical; human - 14/15 amino acid residues identical.
MICU1 Blocking Peptide Cat #: BLP-CC322
Size:
40 µg
Anti-MCU Antibody Cat #: ACC-328
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IH, WB
Reactivity:
H, M, R
Immunogen
Peptide CIEQHQLNKERELIER, corresponding to amino acid residues 191-206 of human mitochondrial calcium uniporter (Accession Q8NE86). N-terminus, mitochondrial matrix.
Homology
Rat, mouse – 15/16 amino acid residues identical.
MCU Blocking Peptide Cat #: BLP-CC328
Size:
40 µg
Anti-VDAC Antibody Cat #: AVC-001
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
WB
Reactivity:
M, R
Immunogen
Peptide (C)DGKNVNAGGHK, corresponding to amino acid residues of 264-274 of rat VDAC1 (Accession Q9Z2L0). Intracellular.
Homology
VDAC1: rat, mouse, human identical; VDAC2: rat, mouse, human - 9/11 amino acid residues identical; VDAC3: rat, mouse - 10/11 amino acid residues identical, human - 9/11 amino acid residues identical.
Pan VDAC Blocking Peptide Cat #: BLP-VC001
Size:
40 µg
Antibody Storage & Reconstitution Instructions
Storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Add deionized water, depending on the sample size.
Storage after reconstitution
The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution
100 μl water.
Control antigen storage after reconstitution
-20ºC.
Preadsorption control
1 μg peptide per 1 μg antibody.
Note
A mouse monoclonal antibody (#ALM-051) and guinea pig polyclonal antibodies (#APC-101-GP & #APC-021-GP) are included in this Explorer Kit. Please take into account when reacting with a secondary antibody.
For research purposes only, not for human use
