Overview
Alternative Name ML133 HCl, N-(4-methoxybenzyl)-1-(naphthalen-1-yl)methanamine hydrochloride
Lyophilized Powder yes
Source Synthetic
MW: 313.82
Purity: >97%
Effective concentration 1-20 µM.
Structure
Chemical name 1-(4-methoxyphenyl)-~{N}-(naphthalen-1-ylmethyl)methanamine; hydrochloride.
Molecular formula C19H19NO · HCl.
CAS No.: 1222781-70-5
PubChem CID 44247466
Activity ML133 hydrochloride selectively inhibits Kir2 inward-rectifier potassium channels with IC50 values of 1.8, 2.8, 2.9 and 4.0 μM for Kir2.1, Kir2.6, Kir2.2 and Kir2.3, respectively (at pH 7.4), and with little or no effect on Kir4.1, Kir7.1 or Kir1.1 channels.1
References-Activity
- Wang, H.R. et al. (2011) ACS Chem. Biol. 6, 845.
Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Solubility Soluble to 100 mM in DMSO. Spin down all products before handling.
Storage of solutions Store at -20°C for several months. It is recommended to aliquot stock solutions to prevent repeated thawing and freezing.
Our bioassay
Alomone Labs ML133 hydrochloride blocks Kir2.1 channels expressed in HEK293 cells.Top: Representative Kir2.1 current traces before (blue) and after (red) application of 10 µM ML133 hydrochloride (#M-275), recorded in pH 8.5 (left) or pH 7.4 (right). Currents were evoked from a holding potential of 0 mV by a 500 ms step to -100 mV, followed by a 500 ms ramp from -100 mV to 100 mV.
Bottom: Corresponding time course of Kir2.1 current inhibition by 10 µM ML133 hydrochloride, as indicated (bar) in pH 8.5 (left) or pH 7.4 (right).
Scientific background
ML133 hydrochloride is a selective small molecule that acts as an inhibitor of Kir2 inward rectifier potassium channels. The compound blocks Kir2.1, Kir2.6, Kir2.2 and Kir2.3 and shows little or no effect on other Kir channels1. ML133 hydrochloride demonstrates IC50 values of 1.8, 2.8, 2.9 and 4.0 μM for Kir2.1, Kir2.6, Kir2.2 and Kir2.3, respectively at pH 7.41.
Kir channels are a family of membrane proteins that selectively control the permeation of K+ ions across cell membranes. These channels play an important role in establishing the resting membrane potential of excitable cells, regulating pacing in cardiomyocytes and neurons, regulating pancreatic insulin secretion and renal K+ transport2.
Target Kir2 channels
Lyophilized Powder
For research purposes only, not for human use
Last Update: 07/05/2024
Specifications
Citations
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