Overview
Type: Synthetic peptide
Form: Lyophilized powder
Mouse Kv1.7/KCNA7 Blocking Peptide (#BLP-PC063) is the original antigen used for immunization during Anti-Mouse KV1.7 (KCNA7) Antibody (#APC-063) generation. The blocking peptide binds and ‘blocks’ Anti-Mouse Kv1.7/KCNA7 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse heart membranes:1. Anti-Mouse KV1.7 (KCNA7) Antibody (#APC-063), (1:200).
2. Anti-Mouse KV1.7 (KCNA7) Antibody, preincubated with Mouse Kv1.7/KCNA7 Blocking Peptide (#BLP-PC063).
Properties
Sequence
- TTRKAQEIHGKAPG(C), corresponding to amino acid residues 2-15 of mouse KV1.7 (Accession Q17ST2).
Accession (Uniprot) Number Q17ST2
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
