Name: Native mouse NGF 2.5S protein (>95%)
Brand: Alomone Labs
SKU: N-100

Overview

Cat #: N-100

Alternative Name Nerve Growth Factor 2.5S

Lyophilized Powder yes

Origin Native protein isolated from mouse submaxillary glands.

MW: 26 kDa

Purity: >95% (HPLC)

Form Lyophilized from a 0.2 µm filtered solution.

Effective concentration EC₅₀ = 0.7 nM.

CAS No.: 93928-24-6

Activity NGF is purified in three forms: the 7S, 2.5S and β. The 2.5S form is 9 amino acids shorter than the β form because of proteolysis that occurs during the purification process¹. NGF has been shown to regulate neuronal survival, development, function and plasticity². The biological effects of NGF are mediated by two receptors: TrkA, which is specific for NGF, and p75^NTR, which binds all the neurotrophins³.

References-Activity

Bocchini, V. and Angeletti, P.U. (1969) Proc. Natl. Acad. Sci. U.S.A. 64, 787.
Huang, E.J. and Reichardt, L.F. (2001) Annu. Rev. Neurosci. 24, 677.
Teng, K.K. and Hempstead, B.L. (2004) Cell Mol. Life Sci. 61, 35.

Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Solubility Sterile water, DMEM at a concentration of at least 10 μg/ml. Centrifuge all product preparations before use (10000 x g 5 min). Repeated freezing/thawing might result in loss of activity.

Storage of solutions Up to one week at 4°C or four-six weeks at -70°C.

Our bioassay

Alomone Labs Native mouse NGF 2.5S protein (>95%) promotes neurite outgrowth and survival in PC12 cells.
Upper panel: PC12 cells were grown in absence of serum and in the presence of varying concentrations of Native mouse NGF 2.5S protein (>95%) (#N-100), the cell survival after 24 h was measured by XTT method and calculated as a relative percentage of the control and plotted against concentrations. Lower panel: PC12 cells were grown on collagen-coated plates in absence (1) or presence (2) of 4 nM Native mouse NGF 2.5S protein (>95%). The development of neurites over a period of 6 days was visualized by Methylene Blue staining.

References - Scientific background

Roux, P.P. and Barker P.A. (2002) Prog. Neurobiol. 67, 203.
Levi-Montalcini, R. (1966) Harvey Lect. 60, 217.
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Bocchini, V. and Angeletti, P.U. (1969) Proc. Natl. Acad. Sci. U.S.A. 64, 787.
McDonald, N.Q. et al. (1991) Nature 354, 411.
Huang, E.J. and Reichardt, L.F. (2001) Annu. Rev. Neurosci. 24, 677.
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Teng, K.K. and Hempstead, B.L. (2004) Cell Mol. Life Sci. 61, 35.
Darling, T. and Shooter, E.M. (1984) "Methods for preparation and assay of nerve growth factor", Cell Culture Methods for Molecular and Cellular Biology, vol. 4 (Eds. D. W. Barnes, D.A. Sinbasku, and G.H. Sato), pp. 79-83, Alan R. Liss, New York.

Scientific background

The neurotrophins ("neuro" means nerve and "trophe" means nutrient) are a family of soluble, basic growth factors which regulate neuronal development, maintenance, survival and death in the CNS and PNS.¹

NGF, the first member of the family to be discovered, was originally purified as a factor able to support survival of sympathetic and sensory spinal neurons in culture.² It is synthesized and secreted by sympathetic and sensory target organs and provides trophic support to neurons as they reach their final target.³

Neurotrophin secretion increases in the nervous system following injury. Schwann cells, fibroblasts, and activated mast cells normally synthesize NGF constitutively, however direct trauma and induction of cytokines combine to increase neurotrophin production in these cells after injury.⁴

NGF is purified in three forms: the 7S, 2.5S and β. The 7S (130 kDa) form occurs naturally in mouse submaxillary glands, and is a multimeric protein composed of two α, one β and two γ subunits. The name is derived from its sedimentation co-efficient, 7S.

The biologically active subunit is the β, which is a 26 kDa dimer composed of two identical 120 amino acid chains held together by hydrophobic interactions.⁵ The 2.5S form is 9 amino acids shorter than the β form because of proteolysis that occurs during the purification process.⁶ The structural hallmark of all the neurotrophins is the characteristic arrangement of the disulfide bridges known as the cysteine knot, which has been found in other growth factors such as Platelet-derived growth factor.⁷ There is a 95.8% homology between the rat and mouse forms, and a 85% homology between the human and mouse.

NGF has been shown to regulate neuronal survival, development, function and plasticity.⁸ Recently, involvement of NGF in processes not involving neuronal cells has been shown, such as asthma,⁹ psoriasis¹⁰ and wound healing.¹¹ The biological effects of NGF are mediated by two receptors: TrkA, which is specific for NGF, and p75^NTR, which binds all the neurotrophins.¹²

Peptide Content: 100%

Lyophilized Powder

Native mouse NGF 2.5S protein (>95%) (#N-100) is a highly pure, natural, and biologically active protein.

Certificate of Analysis SDS

Image & Title
Alomone Labs Native mouse NGF 2.5S protein induces neurite outgrowth in PC12 cells.Neurite outgrowth in PC12 cells was induced by the addition of 50 ng/ml Native mouse NGF 2.5S protein (>95%) (#N-100) or Native mouse NGF 2.5S protein (99%) (#N-240) for 24 hrs.Adapted from Terada, K. et al. (2014) PLoS ONE 9, e93223. with permission of PLoS.

For research purposes only, not for human use

Last Update: 23/05/2024

Applications

Citations

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Native mouse NGF 2.5S protein (>95%)

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