Cat #: BLP-MR061
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- (C)KSHRLQESLGAVLGR, corresponding to amino acid residues 285-299 of rat MRGPRD (Accession Q7TN41).
Accession (Uniprot) Number Q7TN41
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat dorsal root ganglion (lanes 1 and 4), mouse testis (lanes 2 and 5) and rat testis (lanes 3 and 6) lysates:1-3. Anti-MRGPRD (GPCR TGR7) Antibody (#AMR-061), (1:400).
4-6. Anti-MRGPRD (GPCR TGR7) Antibody, preincubated with MRGPRD/GPCR TGR7 Blocking Peptide (#BLP-MR061). - Rat DRG sections (1:200).
- Western blot analysis of rat dorsal root ganglion lysates:1. Guinea Pig Anti-MRGPRD (GPCR TGR7) Antibody (#AMR-061-GP), (1:500).
2. Guinea Pig Anti-MRGPRD (GPCR TGR7) Antibody, preincubated with MRGPRD (GPCR TGR7) Blocking Peptide (BLP-MR061). - Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) testis membranes:1-2. Guinea Pig Anti-MRGPRD (GPCR TGR7) Antibody (#AMR-061-GP), (1:800).
3-4. Guinea Pig Anti-MRGPRD (GPCR TGR7) Antibody, preincubated with MRGPRD (GPCR TGR7) Blocking Peptide (BLP-MR061).
Last update: 02/01/2024