Overview
Type: Synthetic peptide
Form: Lyophilized powder
MRGPRE Blocking Peptide (#BLP-MR062) is the original antigen used for immunization during Anti-MRGPRE Antibody (#AMR-062) generation. The blocking peptide binds and ‘blocks’ Anti-MRGPRE primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat dorsal root ganglion lysate (lanes 1 and 4), mouse (lanes 2 and 5) and rat (lanes 3 and 6) brain membranes:1-3. Anti-MRGPRE Antibody (#AMR-062), (1:400).
4-6. Anti-MRGPRE Antibody, preincubated with MRGPRE Blocking Peptide (#BLP-MR062).
Western blot analysis of human U-87 MG glioblastoma:1. Anti-MRGPRE Antibody (#AMR-062), (1:400).
2. Anti-MRGPRE Antibody, preincubated with MRGPRE Blocking Peptide (#BLP-MR062).
Expression of MRGE in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-MRGPRE Antibody (#AMR-062), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. MRGE staining (green) in hippocampal dentate gyrus is detected in astrocytes (arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)RVERGPERHQPRG, corresponding to amino acid residues 197 - 209 of rat MRGPRE (Accession Q7TN40).
Accession (Uniprot) Number Q7TN40
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
