Cat #: AK-206_KIT
Size: 12 Vials
Form: Lyophilized
Anti-CHRM1 Antibody
Cat #: AMR-001
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, WB
Reactivity:
H, M, R
Immunogen
GST fusion protein with the sequence GSETPGKGGGSSSSSERSQPGAEGSPETPPGRCCRCCRAPRLLQAYSWKEEEEEDEGSMESLTSSEGEEPGSEVVIKMPMVDPEAQAPTKQPPRSSPNTVKRPTKKGRDRAGKGQKPRGKEQLAKRK, corresponding to amino acid residues 227-353 of human M1 (Accession P11229). 3rd intracellular loop.
Homology
Macaca mulatta - 125/127 amino acid residues identical; mouse - 124/127 amino acid residues identical; pig - 124/127 amino acid residues identical; rat - 123/127 amino acid residues identical; gerbil - 123/127 amino acid residues identical.
CHRM1 Blocking Peptide
Cat #: BLP-MR001
Anti-CHRM1 (443-458) Antibody
Cat #: AMR-010
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)RKIPKRPGSVHRTPSR, corresponding to amino acid residues 443-458 of human M1 muscarinic receptor (Accession P11229). Intracellular, C-terminus.
Homology
Mouse, rat - identical.
CHRM1 (443-458) Blocking Peptide
Cat #: BLP-MR010
Anti-CHRM2 Antibody
Cat #: AMR-002
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
GST fusion protein with the sequence VANQDPVSPSLVQGRIVKPN NNNMPSSDDGLEHNKIQNGKAPRDPVTENCVQGEEKESSNDSTSV SAVASNMRDDEITQDENTVSTSLGHSKDENSKQTCIRIGTKTPKS DSCTPTNTTVEVVGSSGQNGDE, corresponding to amino acid residues 225-356 of human M2 (Accession P08172). 3rd intracellular loop.
Homology
Chimpanzee, gorilla - identical; orangutan - 130/132 amino acid residues identical; pig - 122/132 amino acid residues identical; rat - 118/132 amino acid residues identical; mouse - 117/132 amino acid residues identical; dog - 122/132 amino acid residues identical.
CHRM2 Blocking Peptide
Cat #: BLP-MR002
Anti-CHRM3 Antibody
Cat #: AMR-006
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)TLAKRFALKTRSQITKRKR, corresponding to amino residues 461-479 of rat M3 (Accession P08483). 3rd intracellular loop.
Homology
Human, mouse, bovine, pig - identical.
CHRM3 Blocking Peptide
Cat #: BLP-MR006
Anti-CHRM4 Antibody
Cat #: AMR-004
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide(C) RTLNPASKWSK, corresponding to amino acid residues 330-340 of rat M4 muscarinic receptor (Accession P08485). 3rd intracellular loop.
Homology
Mouse - identical; human - 9/12 amino acid residues identical.
CHRM4 Blocking Peptide
Cat #: BLP-MR004
Anti-CHRM5 (extracellular) Antibody
Cat #: AMR-005
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IFC, LCI, WB
Reactivity:
H, R
Immunogen
Peptide (C)HNATTVNGTPVNHQPLER, corresponding to amino acid residues 7-24 of human M5 muscarinic receptor (Accession P08912). Extracellular, N-terminus.
Homology
Mouse - 16/18 amino acid residues identical; rat - 14/18 amino acid residues identical.
CHRM5 (extracellular) Blocking Peptide
Cat #: BLP-MR005
Antibody Storage & Reconstitution Instructions
Storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Add deionized water, depending on the sample size.
Storage after reconstitution
The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution
100 μl water.
Control antigen storage after reconstitution
-20ºC.
Preadsorption control
1 μg peptide per 1 μg antibody.
For research purposes only, not for human use
