Overview
Type: Synthetic peptide
Form: Lyophilized powder
N-Cadherin Blocking Peptide (#BLP-NR082) is the original antigen used for immunization during Anti-N-Cadherin Antibody (#ANR-082) generation. The blocking peptide binds and ‘blocks’ Anti-N-Cadherin primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain (lanes 1 and 5), mouse heart (lanes 2 and 6), rat heart (lanes 3 and 7) and human brain neuroblastoma (SH-SY5Y) (lanes 4 and 8) cell lysates:1-4. Anti-N-Cadherin Antibody (#ANR-082), (1:200).
5-8. Anti-N-Cadherin Antibody, preincubated with N-Cadherin Blocking Peptide (#BLP-NR082).
Expression of N-Cadherin in mouse cortexImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-N-Cadherin Antibody (#ANR-082), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. N-Cadherin immunoreactivity (green) appears in apical dendrites (horizontal arrows) and in the soma (vertical arrows) of pyramidal neurons. B. Pre-incubation of the antibody with N-Cadherin Blocking Peptide (BLP-NR082), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)DTVEPDAIKPVGIR, corresponding to amino acid residues 794-807 of mouse CDH2 (Accession P15116).
Accession (Uniprot) Number P15116
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
