Overview
Type: Synthetic peptide
Form: Lyophilized powder
Na+/H+ Exchanger 2/NHE-2 Blocking Peptide (#BLP-NX002) is the original antigen used for immunization during Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody (#ANX-002) generation. The blocking peptide binds and ‘blocks’ Anti-Na+/H+ Exchanger 2/NHE-2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat kidney membranes (lanes 1 and 4), mouse kidney lysates (lanes 2 and 5) and rat stomach lysates (lanes 3 and 6):1-3. Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody (#ANX-002), (1:200).
4-6. Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody, preincubated with Na+/H+ Exchanger 2/NHE-2 Blocking Peptide (#BLP-NX002).
Western blot analysis of human Colo-205 colorectal carcinoma cell lysates:1. Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody (#ANX-002), (1:200).
2. Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody, preincubated with Na+/H+ Exchanger 2/NHE-2 Blocking Peptide (#BLP-NX002).
Expression of Na+/H+ exchanger 2 in rat colonImmunohistochemical staining of rat colon sections (paraffin-embedded) using Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody (#ANX-002), (1:50). A. NHE-2 staining is shown in red. B. Cell nuclei were labeled with DAPI (blue). C. Merge of the two images.
Properties
Sequence
- (C)RASEPGNRKGRLGNEK, corresponding to amino acid residues 797-812 of rat NHE-2 (Accession P48763).
Accession (Uniprot) Number P48763
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
