Overview
Cat #: BLP-NX033
Type: Synthetic peptide
Form: Lyophilized powder
Na+/H+ Exchanger 3/NHE-3 Blocking Peptide (#BLP-NX033) is the original antigen used for immunization during Anti-Na+/H+ Exchanger 3 (NHE-3) Antibody (#ANX-033) generation. The blocking peptide binds and ‘blocks’ Anti-Na+/H+ Exchanger 3/NHE-3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat small intestine lysate:1. Anti-Na+/H+ Exchanger 3 (NHE-3) Antibody (#ANX-033), (1:200).
2. Anti-Na+/H+ Exchanger 3 (NHE-3) Antibody, preincubated with Na+/H+ Exchanger 3/NHE-3 Blocking Peptide (#BLP-NX033). - Western blot analysis of human Colo-205 colon adenocarcinoma cell lysate:1. Anti-Na+/H+ Exchanger 3 (NHE-3) Antibody (#ANX-033), (1:200).
2. Anti-Na+/H+ Exchanger 3 (NHE-3) Antibody, preincubated with Na+/H+ Exchanger 3/NHE-3 Blocking Peptide (#BLP-NX033).
Properties
Sequence
- (C)EQREPKLNEKLHGR, corresponding to amino acid residues 466-479 of rat NHE-3 (Accession P26433).
Accession (Uniprot) Number P26433
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.