Name: Na+/H+ Exchanger 3/NHE-3 Blocking Peptide
Brand: Alomone Labs
SKU: BLP-NX033

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Cat #: BLP-NX033

Size: 40 µg

Type: Synthetic peptide

Form: Lyophilized powder

Applications: wb, ihc

Application key:

WB- Western blot, IHC- Immunohistochemistry

For research purposes only. not for human use

Properties

Sequence

(C)EQREPKLNEKLHGR, corresponding to amino acid residues 466-479 of rat NHE-3 (Accession P26433).

Accession (Uniprot) Number P26433

Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.

Purity >70%

Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.

Reconstitution 100 µl double distilled water (DDW).

Storage After Reconstitution -20°C.

Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.

Standard Quality Control Of Each Lot Western blot analysis.

Applications

Demonstration of Pre-adsorption control

Western blot analysis of rat small intestine lysate:
1. Anti-Na⁺/H⁺ Exchanger 3 (NHE-3) Antibody (#ANX-033), (1:200).
2. Anti-Na⁺/H⁺ Exchanger 3 (NHE-3) Antibody, preincubated with Na+/H+ Exchanger 3/NHE-3 Blocking Peptide (#BLP-NX033).
Western blot analysis of human Colo-205 colon adenocarcinoma cell lysate:
1. Anti-Na⁺/H⁺ Exchanger 3 (NHE-3) Antibody (#ANX-033), (1:200).
2. Anti-Na⁺/H⁺ Exchanger 3 (NHE-3) Antibody, preincubated with Na+/H+ Exchanger 3/NHE-3 Blocking Peptide (#BLP-NX033).

Last update: 02/01/2024

Specific Use it with:

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat NHE-3. Anti-Na⁺/H⁺ Exchanger 3 (NHE-3) Antibody (#ANX-033) can be used in western blot analysis. It has been designed to recognize NHE-3 from human, mouse and rat samples.

Anti-Na⁺/H⁺ Exchanger 3 (NHE-3) Antibody (#ANX-033)