Overview
Type: Synthetic peptide
Form: Lyophilized powder
Na+/H+ Exchanger 5/NHE-5 Blocking Peptide (#BLP-NX005) is the original antigen used for immunization during Anti-Na+/H+ Exchanger 5 (NHE-5) Antibody (#ANX-005) generation. The blocking peptide binds and ‘blocks’ Anti-Na+/H+ Exchanger 5/NHE-5 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-Na+/H+ Exchanger 5 (NHE-5) Antibody (#ANX-005), (1:200).
3,4. Anti-Na+/H+ Exchanger 5 (NHE-5) Antibody, preincubated with Na+/H+ Exchanger 5/NHE-5 Blocking Peptide (#BLP-NX005).
Properties
Sequence
- CAEKELPWKSGQGD, corresponding to amino acid residues 759-772 of rat NHE-5 (Accession Q9Z0X2).
Accession (Uniprot) Number Q9Z0X2
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
