Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)EHLGVPENERRTTK, corresponding to amino acid residues 515-528 of human NHE-6 (Accession Q92581).
Accession (Uniprot) Number Q92581
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 3) and new born rat brain membranes (lanes 2 and 4):1,2. Anti-Na+/H+ Exchanger 6 (NHE-6) Antibody (#ANX-006), (1:200).
3,4. Anti-Na+/H+ Exchanger 6 (NHE-6) Antibody, preincubated with Na+/H+ Exchanger 6/NHE-6 Blocking Peptide (#BLP-NX006).
Western blot analysis of mouse brain lysate:1. Anti-Na+/H+ Exchanger 6 (NHE-6) Antibody (#ANX-006), (1:200).
2. Anti-Na+/H+ Exchanger 6 (NHE-6) Antibody, preincubated with Na+/H+ Exchanger 6/NHE-6 Blocking Peptide (#BLP-NX006).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:1. Anti-Na+/H+ Exchanger 6 (NHE-6) Antibody (#ANX-006), (1:200).
2. Anti-Na+/H+ Exchanger 6 (NHE-6) Antibody, preincubated with Na+/H+ Exchanger 6/NHE-6 Blocking Peptide (#BLP-NX006).- Rat brain sections (1:200).
Last update: 02/01/2024
