Overview
Type: Synthetic peptide
Form: Lyophilized Powder
Na+/H+ Exchanger 7 (NHE7) (extracellular) Blocking Peptide (#BLP-NX007) is the original antigen used for immunization during Anti-Na+/H+ Exchanger 7 (NHE7) (extracellular) Antibody (#ANX-007) generation. The blocking peptide binds and ‘blocks’ Anti-Na+/H+ Exchanger 7 (NHE7) (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1-2. Anti-Na+/H+ Exchanger 7 (NHE7) (extracellular) Antibody (#ANX-007), (1:200).
3-4. Anti-Na+/H+ Exchanger 7 (NHE7) (extracellular) Antibody, preincubated with Na+/H+ Exchanger 7 (NHE7) (extracellular) Blocking Peptide (BLP-NX007).
Western blot analysis of human U-87 MG glioma cell line lysate:1. Anti-Na+/H+ Exchanger 7 (NHE7) (extracellular) Antibody (#ANX-007), (1:200).
2. Anti-Na+/H+ Exchanger 7 (NHE7) (extracellular) Antibody, preincubated with Na+/H+ Exchanger 7 (NHE7) (extracellular) Blocking Peptide (BLP-NX007).
Expression of NHE7 in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Na+/H+ Exchanger 7 (NHE7) (extracellular) Antibody (#ANX-007), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the mouse hippocampal CA3 region, showed immunoreactivity of NHE7 (green) in the CA3 pyramidal layer (arrows). B. Pre-incubation of the antibody with Na+/H+ Exchanger 7 (NHE7) (extracellular) Blocking Peptide (BLP-NX007), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)EISPGKINNVEQND, corresponding to amino acid residues 158 - 171 of mouse SLC9A7 (Accession Q96T83).
Accession (Uniprot) Number Q96T83
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
