Overview
Alomone Labs is pleased to offer the nAChRβ Antibody Explorer Kit (#AK-232). This Explorer Kit includes antibodies targeting nAChRβ along with their respective peptide control antigen. An ideal tool for screening purposes.
Compounds
| Product Name | Cat # | Size |
|---|---|---|
Anti-Nicotinic Acetylcholine Receptor β1 (CHRNB1) (extracellular) Antibody |
ANC-011 | 1 x 50 µl |
Nicotinic Acetylcholine Receptor β1/CHRNB1 (extracellular) Blocking Peptide |
BLP-NC011 | 1 x 40 µg |
Anti-Nicotinic Acetylcholine Receptor β2 (CHRNB2) (extracellular) Antibody |
ANC-012 | 1 x 50 µl |
Nicotinic Acetylcholine Receptor β2/CHRNB2 (extracellular) Blocking Peptide |
BLP-NC012 | 1 x 40 µg |
Anti-Nicotinic Acetylcholine Receptor β3 (CHRNB3) (extracellular) Antibody |
ANC-013 | 1 x 50 µl |
Nicotinic Acetylcholine Receptor β3/CHRNB3 (extracellular) Blocking Peptide |
BLP-NC013 | 1 x 40 µg |
Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular) Antibody |
ANC-014 | 1 x 50 µl |
Nicotinic Acetylcholine Receptor β4/CHRNB4 (extracellular) Blocking Peptide |
BLP-NC014 | 1 x 40 µg |
Scientific Background
Acetylcholine, released by cholinergic neurons, activates two groups of acetylcholine receptors (AChRs); muscarinic AChRs (mAChRs) which belong to the superfamily of G-protein coupled receptors (GPCRs) and nicotinic AChRs (nAChRs) which belong to the ligand-gated ion channel superfamily. nAChRs also respond to nicotine, hence their name1.
To date, 17 different but related subunits of nAChRs have been identified and cloned. They consist of α subunits (α1-10), which is responsible for the binding of ligands. In fact, this subunit includes a Cys-loop in the first extracellular domain that is required for agonist binding2. The other subunits responsible for making up the active receptor are the β (β1-4), γ, δ and ε subunits3. Structurally, all subunits have the following: a conserved large extracellular N-terminal domain, 3 conserved transmembrane domains, a variable cytoplasmic loop and a fourth transmembrane domain with a short extracellular C-terminal domain. An active nAChR is generally a heteropentamer (homopentamers also exist) of these various subunits organized around a central pore1.
- Albuquerque, E.X. et al (2009) Physiol. Rev. 89, 73.
- Karlin, A. et al. (1986) Ann. NY. Acad. Sci. 463, 53.
- Kalamida, D. et al. (2007) FEBS J. 274, 3799.
