Overview
Type: Synthetic peptide
Form: Lyophilized powder
NALCN/VGCNL1 (extracellular) Blocking Peptide (#BLP-SC022) is the original antigen used for immunization during Anti-NALCN/VGCNL1 (extracellular) Antibody (#ASC-022) generation. The blocking peptide binds and ‘blocks’ Anti-NALCN/VGCNL1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 4), mouse heart (lanes 2 and 5) and human SH-SYS5 brain neuroblastoma cell (lanes 3 and 6) lysates:1-3. Anti-NALCN/VGCNL1 (extracellular) Antibody (#ASC-022), (1:200).
4-6. Anti-NALCN/VGCNL1 (extracellular) Antibody, preincubated with NALCN/VGCNL1 (extracellular) Blocking Peptide (#BLP-SC022).
Expression of NALCN in rat hypothalamusImmunohistochemical staining of rat hypothalamus using Anti-NALCN/VGCNL1 (extracellular) Antibody (#ASC-022). A. NALCN (green) staining appears in the supra-optic nucleus (SON). B. DAPI is used as the counterstain (blue). White arrows point at the sharp border between the SON and optic tract (OT). C. Merged images of A and B.
Properties
Sequence
- (C)SMMFESPFRRVMH, corresponding to amino acid residues 902-914 of rat NALCN (Accession Q6Q760).
Accession (Uniprot) Number Q6Q760
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
