Cat #: ESD-002_PACK
Size: 11 Vials
Form: Lyophilized
Antibodies
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
Anti-SCN2A (NaV1.2) Antibody Cat #: ASC-002
Size:
0.2 ml
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)ASAESRDFSGAGGIGVFSE, corresponding to amino acid residues 467-485 of rat NaV1.2 (Accession P04775). Intracellular loop between domains I and II.
Homology
Homology Mouse, Human - identical.
NaV1.3 – 12 out of 19 amino acid residues identical
NaV1.3 – 12 out of 19 amino acid residues identical
Anti-SCN2A (NaV1.2)-ATTO Fluor-594 Antibody Cat #: ASC-002-AR
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IF, IH
Reactivity:
H, M, R
Immunogen
Peptide (C)ASAESRDFSGAGGIGVFSE, corresponding to amino acid residues 467-485 of rat NaV1.2 (Accession P04775). Intracellular loop between domains I and II.
Homology
Human, mouse – identical.
Label
ATTO-594. Maximum absorption 601 nm; maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label is related to the Rhodamine dyes and can be used with filters used to detect Texas Red and Alexa-594.
Guinea pig Anti-SCN2A (NaV1.2) Antibody Cat #: ASC-002-GP
Size:
0.2 ml
Host: Guinea pig
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)ASAESRDFSGAGGIGVFSE, corresponding to amino acid residues 467-485 of rat NaV1.2 (Accession P04775). Intracellular loop between domains I and II.
Homology
Human - identical.
NaV1.3 - 12/15 amino acid residues identical.
NaV1.3 - 12/15 amino acid residues identical.
Pharmacological Reagents
α-Pompilidotoxin Cat #: P-170
Size:
10 mg
MW: 1529 Da
Purity: >98% (HPLC)
Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
10-100 µM.
Activity
α-Pompilidotoxin slows NaV channel inactivation without changing the peak current-voltage relationship or the activation time course of the TTX-sensitive Na+ currents in the neuromuscular synapse1-3.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to one week at 4°C or three months at -20°C.
Ceratotoxin-1 Cat #: STC-680
Size:
0.1 mg
MW: 4044 Da
Purity: >99% (HPLC)
Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
1-900 nM.
Activity
Ceratotoxin-1 is a NaV channel blocker1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any other aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.
Hainantoxin-IV Cat #: STH-130
Size:
0.1 mg
MW: 3987 Da
Purity: >98% (HPLC)
Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
20-250 nM.
Activity
Neural TTX-sensitive voltage-gated Na+ channel inhibitor1,2.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any other aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.
Phrixotoxin-3 Cat #: STP-720
Size:
0.1 mg
MW: 4060 Da
Purity: >99% (HPLC)
Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
0.5-300 nM.
Activity
Phrixotoxin-3 specifically and reversibly blocks NaV1.21.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10,000 x g 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.
ATX-II Cat #: STA-700
Size:
0.1 mg
MW: 4935 Da
Purity: >98% (HPLC)
Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
10-100 nM.
Activity
ATX-II potently modulates voltage-gated Na+ channel gating kinetics by delaying its inactivation and prolonging the action potential of excitable membranes. ATX-II has been used as a powerful activator of TTX-sensitive and insensitive Na+ channels in various excitable tissue and cell types1-9.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.
Antibody Storage & Reconstitution Instructions
Storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Add deionized water, depending on the sample size.
Storage after reconstitution
The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution
100 μl water.
Control antigen storage after reconstitution
-20ºC.
Preadsorption control
1 μg peptide per 1 μg antibody.
For more information, please refer to individual certificate of analysis for each product.
Note
A guinea pig polyclonal antibody (#ASC-002-GP) is included in this Research Pack. Please take into account when reacting with a secondary antibody.
For research purposes only, not for human use
