Overview
Type: Synthetic peptide
Form: Lyophilized powder
Nav1.5/SCN5A (1978-2016) Blocking Peptide (#BLP-SC013) is the original antigen used for immunization during Anti-NaV1.5 (SCN5A) (1978-2016) Antibody (#ASC-013) generation. The blocking peptide binds and ‘blocks’ Anti-Nav1.5/SCN5A (1978-2016) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of NaV1.5 in NaV1.5 transfected HEK-293 cells:1. Anti-NaV1.5 (SCN5A) (1978-2016) Antibody (#ASC-013), (1:200).
2. Anti-NaV1.5 (SCN5A) (1978-2016) Antibody, preincubated with Nav1.5/SCN5A (1978-2016) Blocking Peptide (#BLP-SC013).
Expression of NaV1.5 in rat cardiac muscleImmunohistochemical staining of NaV1.5 in rat myocardium paraffin-embedded section using Anti-NaV1.5 (SCN5A) (1978-2016) Antibody (#ASC-013), (1:100). Staining is specific for cardiomyocytes while smooth muscles cells in the artery walls are negative (red arrows). Hematoxilin is used as the counterstain.
Properties
Sequence
- GST fusion protein with amino acid residues 1978-2016 of human NaV1.5 (Accession Q14524).
Accession (Uniprot) Number Q14524
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 μl PBS.
Concentration After Reconstitution 1.2 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 3 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
