Overview
Type: Synthetic peptide
Form: Lyophilized powder
Nav1.5/SCN5A (493-511) Blocking Peptide (#BLP-SC005) is the original antigen used for immunization during Anti-NaV1.5 (SCN5A) (493-511) Antibody (#ASC-005) generation. The blocking peptide binds and ‘blocks’ Anti-Nav1.5/SCN5A (493-511) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat heart membranes:1. Anti-NaV1.5 (SCN5A) (493-511) Antibody (#ASC-005), (1:200).
2. Anti-NaV1.5 (SCN5A) (493-511) Antibody, preincubated with Nav1.5/SCN5A (493-511) Blocking Peptide (#BLP-SC005).
Western blot analysis of NaV1.5 in NaV1.5 transfected HEK-293 cells:1. Anti-NaV1.5 (SCN5A) (493-511) Antibody (#ASC-005), (1:200).
2. Anti-NaV1.5 (SCN5A) (493-511) Antibody, preincubated with Nav1.5/SCN5A (493-511) Blocking Peptide (#BLP-SC005).
Expression of NaV1.5 in rat heartImmunohistochemical staining of NaV1.5 in rat myocardium paraffin-embedded sections using Anti-NaV1.5 (SCN5A) (493-511) Antibody (#ASC-005), (1:100). Staining is specific for cardiomyocytes while smooth muscles cells in the small vessels are negative (red arrows). Hematoxilin is used as the counterstain.
Expression of NaV1.5 in mouse heartImmunohistochemical staining of mouse heart sections using Anti-NaV1.5 (SCN5A) (493-511) Antibody (#ASC-005), (1:200). NaV1.5 staining (green) is part of the striate morphology. Nuclei are stained with DAPI (blue).
Western blot analysis of human-NaV1.5 transfected HEK-293 cell lysate (lanes 1 and 3) and rat heart membrane protein (lanes 2 and 4):1,2. Guinea pig Anti-NaV1.5 (SCN5A) Antibody (#ASC-005-GP), (1:200).
3,4. Guinea pig Anti-NaV1.5 (SCN5A) Antibody, preincubated with Nav1.5/SCN5A (493-511) Blocking Peptide (#BLP-SC005).
Expression of NaV1.5 in rat heartImmunohistochemical staining of rat heart frozen sections using Guinea pig Anti-NaV1.5 (SCN5A) Antibody (#ASC-005-GP). NaV1.5 staining (red) appears in cardiomyocytes and in intercalated discs (arrows). Nuclei are stained using DAPI (blue).- Human heart sections (1:200) (Cheng, J. et al. (2017) Sci. Rep. 7, 42953.).
Properties
Sequence
- DRLPKSDSEDGPRALNQLS(C), corresponding to amino acid residues 493-511 of rat NaV1.5 (Accession P15389).
Accession (Uniprot) Number P15389
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
