Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- DRLPKSDSEDGPRALNQLS(C), corresponding to amino acid residues 493-511 of rat NaV1.5 (Accession P15389).
Accession (Uniprot) Number P15389
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat heart membranes:1. Anti-NaV1.5 (SCN5A) (493-511) Antibody (#ASC-005), (1:200).
2. Anti-NaV1.5 (SCN5A) (493-511) Antibody, preincubated with Nav1.5/SCN5A (493-511) Blocking Peptide (#BLP-SC005).
Western blot analysis of NaV1.5 in NaV1.5 transfected HEK-293 cells:1. Anti-NaV1.5 (SCN5A) (493-511) Antibody (#ASC-005), (1:200).
2. Anti-NaV1.5 (SCN5A) (493-511) Antibody, preincubated with Nav1.5/SCN5A (493-511) Blocking Peptide (#BLP-SC005).- Rat heart paraffin sections, and mouse heart sections.
Western blot analysis of human-NaV1.5 transfected HEK-293 cell lysate (lanes 1 and 3) and rat heart membrane protein (lanes 2 and 4):1,2. Guinea pig Anti-NaV1.5 (SCN5A) Antibody (#ASC-005-GP), (1:200).
3,4. Guinea pig Anti-NaV1.5 (SCN5A) Antibody, preincubated with Nav1.5/SCN5A (493-511) Blocking Peptide (#BLP-SC005).- Rat heart sections.
- Human heart sections (1:200) (Cheng, J. et al. (2017) Sci. Rep. 7, 42953.).
Last update: 02/01/2024
