Overview
Type: Synthetic peptide
Form: Lyophilized Powder
NaV1.5 (SCN5A) (extracellular) Blocking Peptide (#BLP-SC045) is the original antigen used for immunization during Anti-NaV1.5 (SCN5A) (extracellular) Antibody (#ASC-045) generation. The blocking peptide binds and ‘blocks’ Anti-NaV1.5 (SCN5A) (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat heart lysate:1. Anti-NaV1.5 (SCN5A) (extracellular) Antibody (#ASC-045), (1:400).
2. Anti-NaV1.5 (SCN5A) (extracellular) Antibody, preincubated with NaV1.5 (SCN5A) (extracellular) Blocking Peptide (BLP-SC045).
Western blot analysis of human MDA-MB-231 breast adenocarcinoma cell line lysate (lanes 1 and 3) and human Jurkat T-cell leukemia cell line lysate (lanes 2 and 4):1-2. Anti-NaV1.5 (SCN5A) (extracellular) Antibody (#ASC-045), (1:200).
3-4. Anti-NaV1.5 (SCN5A) (extracellular) Antibody, preincubated with NaV1.5 (SCN5A) (extracellular) Blocking Peptide (BLP-SC045).
Properties
Sequence
- Peptide CESFNVTGELYWTKVK, corresponding to amino acid residues 1386 - 1401 of mouse SCN5A (Accession Q9JJV9).
Accession (Uniprot) Number Q9JJV9
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
