Cat #: ESD-001_PACK
Size: 14 Vials
Form: Lyophilized
Antibodies
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
Anti-NaV1.7 (SCN9A) Antibody Cat #: ASC-008
Size:
0.2 ml
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)EFTSIGRSR IMGLSE, corresponding to amino acid residues 446-460 of rat NaV1.7 (Accession O08562). Intracellular loop between domains I and II.
Homology
Rabbit - 14/15 amino acid residues identical; human, mouse - 13/15 amino acid residues identical.
Anti-NaV1.7 (SCN9A)-ATTO Fluor-633 Antibody Cat #: ASC-008-FR
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IF, IH
Reactivity:
H, M, R
Immunogen
Peptide (C)EFTSIGRSR IMGLSE, corresponding to amino acid residues 446-460 of rat NaV1.7 (Accession O08562). Intracellular loop between domains I and II.
Homology
Rabbit - 14/15 amino acid residues identical; human, mouse - 13/15 amino acid residues identical.
Label
ATTO-633. Maximum absorption 629 nm; maximum fluorescence 657 nm. The fluorescence is excited most efficiently in the 610 - 645 nm range. This label is analogous to the dyes Alexa 647, Alexa 633 and Cy5 and can be used for direct flow cytometry (FACS) using the He:Ne laser.
Guinea pig Anti-NaV1.7 (SCN9A) Antibody Cat #: ASC-008-GP
Size:
0.2 ml
Host: Guinea pig
Type: Polyclonal
Application:
WB
Reactivity:
R
Immunogen
Peptide (C)EFTSIGRSR IMGLSE, corresponding to amino acid residues 446-460 of rat NaV1.7 (Accession O08562). Intracellular loop between domains I and II.
Homology
Human, mouse - 13/15 amino acid residues identical.
Anti-NaV1.7 (SCN9A) (extracellular) Antibody Cat #: ASC-027
Size:
0.2 ml
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, LCI, WB
Reactivity:
R
Immunogen
Peptide (C)EKEGQTEYMDY(K), corresponding to amino acid residues 1533-1543 of rat NaV1.7 (Accession O08562). 1st extracellular loop, domain IV.
Homology
Mouse - 8/11 amino acid residues identical.
Human - Not recommended to use with human samples.
Human - Not recommended to use with human samples.
Pharmacological Reagents
N-Me-aminopyrimidinone 9 Cat #: N-310
Size:
10 mg
Source: Synthetic
MW: 303.4
Purity: >98%
Effective concentration
10 nM - 1 µM.
Activity
N-Me-aminopyrimidinone 9 is a NaV1.7 channel blocker with IC50 value of 100 nM for NaV1.7 channels in their inactivated state1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
50 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.
PF-04856264 Cat #: P-265
Size:
10 mg
Source: Synthetic
MW: 437.5
Purity: >98% (HPLC)
Effective concentration
10 nM - 1 µM.
Activity
PF-04856264 is a blocker of NaV1.7 with an IC50 value of 28 nM for its inactivated state1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
50 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.
ProTx-III Cat #: STP-150
Size:
0.1 mg
MW: 3802 Da
Purity: >98% (HPLC)
Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
1-250 nM
Activity
ProTx-III is a potent blocker of NaV1.7 voltage-gated Na+ channels1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any other aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.
Pyrrolopyrimidine 48 Cat #: P-275
Size:
10 mg
Source: Synthetic
MW: 385.46
Purity: >99% (HPLC)
Effective concentration
50 nM - 20 µM.
Activity
Pyrrolopyrimidine 48 is a blocker of NaV1.7 channel with an IC50 value of 0.11 µM. It also blocks NaV1.5 channel with an IC50 of 0.16 µM1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
50 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.
XEN907 Cat #: X-105
Size:
10 mg
Source: Synthetic
MW: 351.1
Purity: >99% (HPLC)
Effective concentration
1 nM - 10 µM.
Activity
XEN907 is a NaV1.7 channel blocker with an IC50 value of 5 nM for the channel in its inactivated state1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
100 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.
ATX-II Cat #: STA-700
Size:
0.1 mg
MW: 4935 Da
Purity: >98% (HPLC)
Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
10-100 nM.
Activity
ATX-II potently modulates voltage-gated Na+ channel gating kinetics by delaying its inactivation and prolonging the action potential of excitable membranes. ATX-II has been used as a powerful activator of TTX-sensitive and insensitive Na+ channels in various excitable tissue and cell types1-9.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.
Antibody Storage & Reconstitution Instructions
Storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Add deionized water, depending on the sample size.
Storage after reconstitution
The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution
100 μl water.
Control antigen storage after reconstitution
-20ºC.
Preadsorption control
1 μg peptide per 1 μg antibody.
For more information, please refer to individual certificate of analysis for each product.
Note
A guinea pig polyclonal antibody (#ASC-008-GP) is included in this Research Pack. Please take into account when reacting with a secondary antibody.
For research purposes only, not for human use
