Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)EFTSIGRSR IMGLSE, corresponding to amino acid residues 446-460 of rat NaV1.7 (Accession O08562).
Accession (Uniprot) Number O08562
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of DRG lysates:1. Anti-NaV1.7 (SCN9A) Antibody (#ASC-008), (1:200).
2. Anti-NaV1.7 (SCN9A) Antibody, preincubated with Nav1.7/SCN9A Blocking Peptide (#BLP-SC008).
Western blot analysis of ND7/23 cell lysate (lanes 1,3) and rat brain membranes (lanes 2,4):1,2. Anti-NaV1.7 (SCN9A) Antibody (#ASC-008), (1:200).
3,4. Anti-NaV1.7 (SCN9A) Antibody, preincubated with Nav1.7/SCN9A Blocking Peptide (#BLP-SC008).- Rat dorsal root ganglion (DRG) frozen section.
Human cervical tissue (1:25) (Hernandez-Plata, E. et al. (2012) Int. J. Cancer 130, 2013.).
Mouse dorsal root ganglia (DRGs) (1:1000) (Amaya, F. et al. (2006) J. Neurosci. 26, 12852.). 
Western blot analysis of rat DRG lysates:1. Guinea pig Anti-NaV1.7 (SCN9A) Antibody (#ASC-008-GP), (1:400).
2. Guinea pig Anti-NaV1.7 (SCN9A) Antibody, preincubated with Nav1.7/SCN9A Blocking Peptide (#BLP-SC008).
Note: addition of 0.5% Tween-20 to the primary antibody solution may strengthen the signal and lower background.
Last update: 02/01/2024
