Cat #: ESD-003_PACK
Size: 10 Vials
Form: Lyophilized
Antibodies
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
Anti-NaV1.8 (SCN10A) Antibody Cat #: ASC-016
Size:
0.2 ml
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
M, R
Immunogen
Peptide (C)EDEVAAKEGNSPGPQ, corresponding to amino acid residues 1943-1956 of rat NaV1.8 (Accession Q63554). Intracellular, C-terminus.
Homology
Mouse - 14/15 amino acid residues identical; human - 7/15 amino acid residues identical.
Anti-NaV1.8 (SCN10A)-ATTO Fluor-594 Antibody Cat #: ASC-016-AR
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IF, IH
Reactivity:
H, R
Immunogen
Peptide (C)EDEVAAKEGNSPGPQ, corresponding to amino acid residues 1943-1956 of rat NaV1.8 (Accession Q63554). Intracellular, C-terminus.
Homology
Mouse - 14/15 amino acid residues identical; human - 7/15 amino acid residues identical.
Label
ATTO-594. Maximum absorption 601 nm; maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label is related to the Rhodamine dyes and can be used with filters used to detect Texas Red and Alexa-594.
Guinea pig Anti-NaV1.8 (SCN10A) Antibody Cat #: ASC-016-GP
Size:
0.2 ml
Host: Guinea pig
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
R
Immunogen
Peptide (C)EDEVAAKEGNSPGPQ, corresponding to amino acid residues 1943-1956 of rat NaV1.8 (Accession Q63554). Intracellular, C-terminus.
Homology
Mouse - 14/15 amino acid residues identical; human - 7/15 amino acid residues identical.
Anti-NaV1.8 (SCN10A) (extracellular) Antibody Cat #: ASC-028
Size:
0.2 ml
Host: Rabbit
Type: Polyclonal
Application:
WB
Reactivity:
M, R
Immunogen
Peptide (C)EDNYLEEKPRVKSVLE, corresponding to amino acid residues 1173-1188 of rat NaV1.8 (Accession Q62968). 1st extracellular loop, domain III.
Homology
Mouse - identical; human – Not recommended for use with human samples.
Pharmacological Reagents
A-803467 Cat #: A-105_50 mg
Size:
50 mg
Source: Synthetic
MW: 357.8
Purity: >99% (HPLC)
Effective concentration
10 nM - 10 μM.
Activity
A-803467 is a potent and selective blocker of the TTX-resistant NaV1.8 channel. A-803467 is a state dependent inhibitor with IC50 of 8 nM at depolarized membrane potential (-40 mV) and 80 nM at -100 mV. As an efficient inhibitor of TTX-resistant currents in DRG neurons, A-803467 was also effective as an analgesic in several rodent models of different pathological pain states1,2. A-803467 was used as a pharmacological tool to demonstrate the expression of NaV1.8 in the heart conduction system and its contribution to QRS interval in the mouse heart3.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.
VSTX3 Cat #: STT-350
Size:
0.1 mg
MW: 4172 Da
Purity: >98% (HPLC)
Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
200-500 nM.
Activity
VSTX3 inhibits voltage-gated Na+ channels NaV1.3, NaV1.7 and NaV1.81.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Soluble in water. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.
Antibody Storage & Reconstitution Instructions
Storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Add deionized water, depending on the sample size.
Storage after reconstitution
The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution
100 μl water.
Control antigen storage after reconstitution
-20ºC.
Preadsorption control
1 μg peptide per 1 μg antibody.
For more information, please refer to individual certificate of analysis for each product.
Note
A guinea pig polyclonal antibody (#ASC-016-GP) is included in this Research Pack. Please take into account when reacting with a secondary antibody.
For research purposes only, not for human use
