Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)EDEVAAKEGNSPGPQ, corresponding to amino acid residues 1943-1956 of rat NaV1.8 (Accession Q63554).
Accession (Uniprot) Number Q63554
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat DRG lysates:1. Anti-NaV1.8 (SCN10A) Antibody (#ASC-016), (1:200).
2. Anti-NaV1.8 (SCN10A) Antibody, preincubated with Nav1.8/SCN10A Blocking Peptide (#BLP-SC016).- Adult rat DRG frozen sections. Human neuromas (1:100) (Black, J.A. et al. (2008) Ann. Neurol. 64, 644.).
Western blot analysis of rat DRG lysate:1. Guinea pig Anti-NaV1.8 (SCN10A) Antibody (#ASC-016-GP), (1:200).
2. Guinea pig Anti-NaV1.8 (SCN10A) Antibody, preincubated with Nav1.8/SCN10A Blocking Peptide (#BLP-SC016).- Rat DRG. Following a broad screen of secondary antibodies, the following was used for this application: #106-485-006 (Jackson ImmunoResearch).
Last update: 02/01/2024
