Overview
Type: Synthetic peptide
Form: Lyophilized powder
NCAM2 (extracellular) Blocking Peptide (#BLP-NR042) is the original antigen used for immunization during Anti-NCAM2 (extracellular) Antibody (#ANR-042) generation. The blocking peptide binds and ‘blocks’ Anti-NCAM2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-NCAM2 (extracellular) Antibody (extracellular) Antibody (#ANR-042), (1:200).
3,4. Anti-NCAM2 (extracellular) Antibody (extracellular) Antibody, preincubated with NCAM2 (extracellular) Blocking Peptide (#BLP-NR042).
Western blot analysis of human LNCaP prostate carcinoma (lanes 5 and 7) and MCF-7 breast adenocarcinoma (lanes 6 and 8) cell lysates:5,6. Anti-NCAM2 (extracellular) Antibody (extracellular) Antibody (#ANR-042), (1:200).
7,8. Anti-NCAM2 (extracellular) Antibody (extracellular) Antibody, preincubated with NCAM2 (extracellular) Blocking Peptide (#BLP-NR042).
Expression of NCAM2 in mouse brainImmunohistochemical staining of mouse free-floating frozen brain sections using Anti-NCAM2 (extracellular) Antibody (#ANR-042). A. In the hippocampal CA3 region, NCAM2 immunoreactivity (green) appears in the pyramidal layer (P, arrows). B. In the olfactory bulb, NCAM2 staining (green) appears in the Mitral cell layer (MCL, arrows). Nuclei are demonstrated using DAPI as the counterstain (blue).
Properties
Sequence
- (C)KEDQWLEKKVQGNKDH, corresponding to amino acid residues 635-650 of mouse NCAM-2 (Accession O35136).
Accession (Uniprot) Number O35136
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
