Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)KEDQWLEKKVQGNKDH, corresponding to amino acid residues 635-650 of mouse NCAM-2 (Accession O35136).
Accession (Uniprot) Number O35136
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-NCAM2 (extracellular) Antibody (extracellular) Antibody (#ANR-042), (1:200).
3,4. Anti-NCAM2 (extracellular) Antibody (extracellular) Antibody, preincubated with NCAM2 (extracellular) Blocking Peptide (#BLP-NR042).
Western blot analysis of human LNCaP prostate carcinoma (lanes 5 and 7) and MCF-7 breast adenocarcinoma (lanes 6 and 8) cell lysates:5,6. Anti-NCAM2 (extracellular) Antibody (extracellular) Antibody (#ANR-042), (1:200).
7,8. Anti-NCAM2 (extracellular) Antibody (extracellular) Antibody, preincubated with NCAM2 (extracellular) Blocking Peptide (#BLP-NR042).- Mouse brain sections.
Last update: 11/02/2024
