Overview
Type: Synthetic peptide
Form: Lyophilized powder
NCX1/SLC8A1 Blocking Peptide (#BLP-NX011) is the original antigen used for immunization during Anti-NCX1 (SLC8A1) Antibody (#ANX-011) generation. The blocking peptide binds and ‘blocks’ Anti-NCX1/SLC8A1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-NCX1 (SLC8A1) Antibody (#ANX-011), (1:200).
3,4. Anti-NCX1 (SLC8A1) Antibody, preincubated with NCX1/SLC8A1 Blocking Peptide (#BLP-NX011).
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) heart lysates:1,2. Anti-NCX1 (SLC8A1) Antibody (#ANX-011), (1:200).
3,4. Anti-NCX1 (SLC8A1) Antibody, preincubated with NCX1/SLC8A1 Blocking Peptide (#BLP-NX011).
Expression of NCX1 in rat heartImmunohistochemical staining of rat heart paraffin-embedded sections using Anti-NCX1 (SLC8A1) Antibody (#ANX-011), (1:100). Hematoxilin is used as the counterstain. NCX1 labeling (brown) appears in the cardiac muscle cells (CM), and not in other parts of the tissue, such as the blood vessel endothelium (E), the connective tissue (CT) and smooth muscle (SM).
Properties
Sequence
- (C)EVDERDQDDEEAR, corresponding to amino acid residues 308-320 of rat NCX1 (Accession Q01728).
Accession (Uniprot) Number Q01728
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
